They now trail the Carthage Eagles by 3 points and if both sides win the remainder of games in Group A the North Africans would be certain of claiming the ticket to the tournament.
However, the Congolese coach Florent Ibenge remains hopeful and believes anything can happen in their favour.
On a list of 23 players he unveiled for their October 7 match with already eliminated Libya, Benik Afobe is nowhere to be found while Jordan Botaka re-emerges after being ignored for months.
Bournemouth striker Benik Afobe, midfielder Remy Mulumba and goalkeeper Nicaise Kudimbana were not invited. But Mbemba, Kakuta, Mubele and Bakambu are all present alongside Luyindama.
Due to political instability in the country, Libya will be hosting the encounter far away from home in Monastir, Tunisia.
Congo will camp in Morocco as from October 2, according to officials.
Goalkeepers: Matampi Vumi Ley (Mazembe), Joel Kiasumbua (Lugano/Switzerland), Nathan Mabula Mabruki (Daring Club Motema Pembe)
Defenders : Jordan Ikoko (Guimgamp/France), Issama Mepko (Mazembe), Gabriel Zakuani (Gilligam/ England), Marcel Tisserand (Wolfsburg/Germany), Fabrice Nsakala (Alanyaspor/Turkey), Glody Ngonda Muzinga (V.Club), Christian Luyindama Nekadio (Standard de Liege/Belgium), Wilfried Moke (Konyaspor/Turkey)
Midfielders : Chancel Mbemba (Newcastle/England), Jacques Maghoma (Birmingham/ England), Merveille Bope Bokadi (Standard de Liege/Belgium), Neeskens Kebano (Fulham/ England), Paul-Jose Mpoku (Standard de Liege/Belgium), Gaël Kakuta (Amiens/France)
Forwards: Jordan Botaka (Saint-Trond/Belgium), Cedric Bakambu (Villareal/Spain), Firmin Mubele (Rennes/France), Jonathan Bolingi (Mouscron/Belgium), Junior Kabananga (FK Astana/Kazakhstan), Chadrac Akolo (VBF Stuttgart/Germany)